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dc.contributor.advisorCanosa, Luis Fabiánspa
dc.contributor.authorCotrino Salcedo, Laura Angélicaspa
dc.coverage.spatialCampus UMNGspa
dc.date.accessioned2016-10-04T22:18:05Z
dc.date.accessioned2019-12-26T21:08:32Z
dc.date.available2016-10-04T22:18:05Z
dc.date.available2019-12-26T21:08:32Z
dc.date.issued2016-02-01
dc.identifier.urihttp://hdl.handle.net/10654/14110
dc.description.abstractLa hormona de crecimiento (GH) controla funciones fisiológicas esenciales en peces teleósteos, incluyendo crecimiento, metabolismo y osmoregulación. En los últimos años se han identificado los receptores de GH (GHR-I y GHR-II) en peces, los cuales se expresan altamente en el hígado. GH, a través de los GHRs, estimula la producción de factores de crecimiento similares a insulina (IGFs), los cuales median las acciones de GH en muchos de los procesos fisiológicos mencionados. En este trabajo se estudió el efecto de GH bovina, insulina, cortisol y estradiol sobre los niveles de ARNm de GHRI, GHRII, IGFI e IGFII en cultivos primarios de hepatocitos de Pejerrey, para lo que, como primera medida, fue necesario la estandarización de dicha metodología para la especie. Diferentes concentraciones de GHb estimulan los niveles de ARNm de GHRI e IGFI, pero no afectan los niveles de ARNm de GHRII e IGFII. Insulina estimula levemente la expresión de GHRI e IGFII, pero no afecta GHRII e IGFI. Cortisol y estradiol por si solos no presentaron acciones estimulantes o inhibitorias sobre la expresión de los genes de estudio. La acción combinada de tanto de GHb e insulina y GHb y cortisol, tiene un efecto negativo sobre la acción de GH, lo que resulta en la reducción de los niveles de ARNm de los genes de interés. GHb y estradiol promueven el aumento de los niveles de ARNm de todos los genes, es decir, E2 tiene un efecto estimulante sobre la acción de GH. Este estudio muestra que la expresión de GHRs e IGFs en hepatocitos de Pejerrey en cultivo primario está diferencialmente regulada por diferentes concentraciones de GH e insulina administradas de manera individual, y por GH y hormonas esteroides administradas en conjunto.spa
dc.description.sponsorshipInstituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECH)spa
dc.description.sponsorshipUniversidad Militar Nueva Granadaspa
dc.format.mimetypeapplication/pdfspa
dc.language.isospaspa
dc.titleEfectos de la hormona de crecimiento (GH), insulina, cortisol y estradiol sobre la expresión de los receptores de hormona de crecimiento (GHRs) y de los factores de crecimiento similares a insulina (IGFs) en hepatocitos de Pejerrey (Odontesthes bonariensis) en cultivo.spa
dc.typeinfo:eu-repo/semantics/bachelorThesisspa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.subject.lembHORMONAS - CRECIMIENTOspa
dc.subject.lembPECES MARINOSspa
dc.subject.lembPECES MARINOS - CRECIMIENTOspa
dc.publisher.departmentFacultad de Ciencias Básicasspa
dc.type.localTrabajo de gradospa
dc.description.abstractenglishGrowth hormone (GH) controls essential physiological functions in teleost fish, including growth, metabolism and osmoregulation. Recent studies have identified GH receptors (GHR-I and GHR-II) in fish, which are highly expressed in the liver. Similarly, GH stimulates production of insulin-like growth factors (IGFs), which mediate the actions of GH in such metabolic processes. In this work we investigated the effect of bovine GH, insulin, cortisol and estradiol on mRNA levels GHRI, GHRII, IGFI and IGFII in primary cultured Pejerrey hepatocytes and as a first step, it was necessary to standardize the methodology for the specie. Different concentrations of GHb stimulate mRNA levels of GHRI and IGFI, but did not affect mRNA levels GHRII and IGFII. Insulin stimulates expression level of GHRI and IGFII, but did not affect GHRII and IGFI. Cortisol and estradiol administered alone showed no stimulatory or inhibitory actions on the expression of the genes studied. Combination of both insulin and GHb, and GHb and cortisol, had a negative effect on the action of GH, resulting in reducing mRNA levels of the genes of interest. GHb and estradiol increased mRNA levels of all genes, ie, E2 has a stimulating effect on GH action. This study shows that GHRs and IGFs gene expression are differentially regulated by different concentrations of GH and insulin administered individually, and GH and steroids hormones administered in combination, in primary culture of Pejerrey hepatocytes.eng
dc.title.translatedEffects of growth hormone (GH), insulin, cortisol and estradiol on the expresion of growth hormone receptors (GHRs) and insulin-like growth factors (IGFs) in cultured Pejerrey (Odontesthes bonariensis) hepatocytesspa
dc.subject.keywordsGrowth hormonespa
dc.subject.keywordsInsulinspa
dc.subject.keywordsCortisolspa
dc.subject.keywordsEstradiolspa
dc.subject.keywordsGH receptorsspa
dc.subject.keywordsInsulin-like growth factorsspa
dc.publisher.programBiología Aplicadaspa
dc.creator.degreenameBiólogospa
dc.description.degreelevelPregradospa
dc.type.versioninfo:eu-repo/semantics/acceptedVersionspa
dc.relation.referencesCanosa, L., Chang, J y Peter, R. 2007. Neuroendocrine control of growth hormone in fish. General and Comparative Endocrinology, 151: 1–26p.spa
dc.relation.referencesCanosa, L., Gomez-Requeni, P. y Cerdá-Reverter, J.M. 2013. Integrative cross-talk between food intake and growth function. Chapter 10, 179-239 p. En: Polakof, S. y Moon, T (Eds.) 2013. Trout: From physiology to conservation, Nova Science Publishers, Inc., New York, USA, 452p.spa
dc.relation.referencesCanosa, L.F., Lin, X., y Peter, R.E. 2002. Regulation of somatostatin gene expression in goldfish brain by sex steroids hormones. Neuroendocrinology. 76: 8-17pspa
dc.relation.referencesChang, JP y Wong, AOL. 2009. Growth Hormone Regulation in Fish: A Multifactorial Model with Hypothalamic, Peripheral and Local Autocrine/Paracrine Signals. p. 151-195. En: Bernier NJ, GVD Kraak, AP Farrel and CJ Brauner (Eds.). Fish Neuroendocrinology. Academic Press, London, 529p.spa
dc.relation.referencesGoméz-Requeni, P., Kraemer, M. y Canosa, L. 2012. Regulation of somatic growth and gene expression of the GH-IGF system and PRP-PACAP by dietary lipid level in early juveniles of a teleost fish, the pejerrey (Odontesthes bonariensis). Journal of comparative physiology, 175 (1): 517-530p.spa
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dc.relation.referencesPierce, A.L., Breves, J.P., Moriyama, S., Hirano, T. y Grau, E.G. 2011. Differential regulation of Igf1 and Igf2 mRNA levels in tilapia hepatocytes: effects of insulin and cortisol on GH sensitivity. Journal of endocrinology, 211: 201-210p.spa
dc.relation.referencesPierce, A.L., Breves, J.P., Moriyama, S., Uchida, K. y Grau, E.G. 2012. Regulation of growth hormone (GH) receptor (GHR1 and GHR2) m RNA level by GH and metabolic hormones in primary cultured tilapia hepatocytes. General and comparative endocrinology, 179: 22-29p.spa
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dc.relation.referencesPierce, A.L., Dickey, J.T., Larsen, D.A., Fukada, H., Swanson, P. y Dickhoff, W.W. 2004. A quantitative real-time RT-PCR assay for salmon IGF-1 mRNA, and its application in the study of GH regulation of IGF-1 gene expression in primary culture of salmon hepatocytes. General and comparative endocrinology, 135: 401-411pspa
dc.relation.referencesPierce, A.L., Fox, B.K., Davis, L.K., Visitacion, N., Kitahashi, T., Hirano, T. y Grau, E.G. 2007. Prolactin receptor, growth hormone receptor, and putative somatolactin receptor in Mozambique tilapia: Tissue specific expression and differential regulation by salinity and fasting. General and comparative endocrinology, 154: 31-40p.spa
dc.relation.referencesPierce, A.L., Fukada, H. y Dickhoff, W.W. 2005. Metabolic hormones modulate the effect of growth hormone (GH) on insulin-like growth factor-I (IGF-I) mRNA level in primary culture of salmon hepatocytes. Journal of endocrinology, 184: 341-349p.spa
dc.relation.referencesPierce, A.L., Shimizu, M., Felli, L., Swanson, P. y Dickhoff, W.W. 2006. Metabolic hormones regulate insulin-like growth factor binding protein-1 mRNA levels in primary cultured salmon hepatocytes; lack of inhibition by insulin. Journal of endocrinology, 191: 379-386p.spa
dc.relation.referencesReindl, K. y Sheridan, M. 2012. Peripheral regulation of the growth hormone-insulin-like growth factor system in fish and other vertebrates. Comparative biochemistry and physiology, 163: 231-245p.spa
dc.relation.referencesSciara, A., Somoza, G.M. y Arranz, S. 2008. Insulin-like growth factor-I of Pejerrey, Odontesthes bonariensis: cDNA characterization, tissue distribution and expression profiles after growth hormone administration. Journal of experimental zoology, 309A: 407-418p.spa
dc.relation.referencesWong, A., Zhou, H., Jiang, y Ko, W. 2006. Feedback regulation of growth hormone synthesis and secretion in fish and the emerging concept of intrapituitary feedback loop. Comparative Biochemistry and Physiology, Part A 144 (2006) 284 – 305.spa
dc.subject.proposalHormona de crecimientospa
dc.subject.proposalInsulinaspa
dc.subject.proposalCortisolspa
dc.subject.proposalEstradiolspa
dc.subject.proposalReceptores de GHspa
dc.subject.proposalFactores de crecimmiento similares a insulinaspa
dc.publisher.grantorUniversidad Militar Nueva Granadaspa


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